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pCvGAPDH-NanoLuc
(Plasmid #255780)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255780 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2931
  • Total vector size (bp) 5198
  • Vector type
    Bacterial Expression ; Chromera velia, Toxoplasma gondii

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NanoLuc
  • Species
    Synthetic
  • Insert Size (bp)
    510
  • Promoter putative C. velia GAPDH promoter (1099 bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site AflII (not destroyed)
  • 5′ sequencing primer pBluescript KS
  • 3′ sequencing primer pBluescript SK
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on pCvGAPDH-mNeonGreen (pBluescript-derived expression vector containing the putative C. velia GAPDH promoter region (1099 bp; VEuPathDB: Cvel_5775), mNeonGreen CDS, a 13-aa linker, and the C. velia GAPDH 3′ UTR (632 bp).

NanoLuc CDS was amplified from pCDH-EF1-Nluc (#73024, Addgene) and inserted between the BglII and AflII sites of pCvGAPDH-mNeonGreen to replace the CDS of mNeonGreen. Intended for expression in C. velia.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCvGAPDH-NanoLuc was a gift from Ke Hu (Addgene plasmid # 255780 ; http://n2t.net/addgene:255780 ; RRID:Addgene_255780)

  • For your References section:

    Transfection of the free-living alga Chromera velia enables direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondii. Tengganu IF, Hu K. J Cell Sci. 2026 Jan 1;139(1):jcs.264400. doi: 10.1242/jcs.264400. Epub 2026 Jan 13. 10.1242/jcs.264400 PubMed 41208444
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