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pET15b_6xHis-A1aY1-superTagRFP
(Plasmid #255802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255802 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A1aY1
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • superTagRFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b_6xHis-A1aY1-superTagRFP was a gift from Kristen Verhey (Addgene plasmid # 255802 ; http://n2t.net/addgene:255802 ; RRID:Addgene_255802)

  • For your References section:

    Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation. Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ. Elife. 2026 Feb 9;14:RP109308. doi: 10.7554/eLife.109308. 10.7554/eLife.109308 PubMed 41662400
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