Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation.
Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ
Elife. 2026 Feb 9;14:RP109308. doi: 10.7554/eLife.109308.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255800 | pC1_superTagRFP-A1aY1 | mammalian expression of the synthetic protein A1aY1 which recognizes the C-terminal tail of alpha-tubulin. Tagged with superTagRFP. Based on Addgene Plasmid #158754. |
| 255801 | pN1_4xCAPGly-mSc3 | mammalian expression of the CAP-Gly domain of CLIP-170 (rat CLIP1 aa 3-484) which recognizes the C-terminal tail of alpha-tubulin. Tagged with mScarlet3. |
| 255804 | pCAGGS_internal PA-TubA1A-IRES-EGFP | mammalian expression of PA-tagged human TubA1A(E254A). The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. An IRES drives expression of EGFP reporter. |
| 255810 | pCAGGS_PA-mEGFP-Tau | mammalian expression of Tau tagged with PA and mEGFP |