Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation.
Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ
Elife. 2026 Feb 9;14:RP109308. doi: 10.7554/eLife.109308.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255800 | pC1_superTagRFP-A1aY1 | mammalian expression of the synthetic protein A1aY1 which recognizes the C-terminal tail of alpha-tubulin. Tagged with superTagRFP. Based on Addgene Plasmid #158754. |
| 255801 | pN1_4xCAPGly-mSc3 | mammalian expression of the CAP-Gly domain of CLIP-170 (rat CLIP1 aa 3-484) which recognizes the C-terminal tail of alpha-tubulin. Tagged with mScarlet3. |
| 255802 | pET15b_6xHis-A1aY1-superTagRFP | bacterial expression and purification of the synthetic protein A1aY1 which recognizes the C-terminal tail of alpha-tubulin. Tagged with superTagRFP. Based on Addgene Plasmid #158753. |
| 255803 | pET15b_6_His-4xCAPGly-mEGFP | bacterial expression and purification of the CAP-Gly domain of CLIP-170 (rat CLIP1 aa 3-484) which recognizes the C-terminal tail of alpha-tubulin. Tagged with mEGFP. |
| 255804 | pCAGGS_internal PA-TubA1A-IRES-EGFP | mammalian expression of PA-tagged human TubA1A(E254A). The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. An IRES drives expression of EGFP reporter. |
| 255805 | pCAGGS_internal PA-TubA1A(E254A) | mammalian expression of PA-tagged human TubA1A(E254A). The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. |
| 255806 | pCAGGS_internal PA-TubA1A | mammalian expression of PA-tagged human TubA1A. The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. |
| 255807 | pCAGGS_internal PA-TubA1A(E254A)-IRES-EGFP | mammalian expression of PA-tagged human TubA1A. The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. An IRES drives expression of EGFP reporter. |
| 255808 | pCAGGS_PA-MAP2-mEGFP | mammalian expression of MAP2 tagged with PA and mEGFP |
| 255809 | pN1_PA-MAP7-mEGFP | mammalian expression of MAP7 tagged with PA and mEGFP |
| 255810 | pCAGGS_PA-mEGFP-Tau | mammalian expression of Tau tagged with PA and mEGFP |
| 255811 | pCAGGS_PA-mEGFP-CAMSAP2 | mammalian expression of CAMSAP2 tagged with PA and mEGFP |
| 255812 | pCAGGS_PA-mEGFP-CAMSAP3 | mammalian expression of CAMSAP3 tagged with PA and mEGFP |
| 255813 | pCAGGS-mEGFP-TubA1A | mammalian expression of TubA1A tagged with mEGFP |