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pN1_4xCAPGly-mSc3
(Plasmid #255801)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255801 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLIP-170 CAP-Gly
  • Alt name
    CLIP1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Clip1 (a.k.a. Rsn, CLIP-170, MGC114234)
  • Tag / Fusion Protein
    • mScarlet3 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN1_4xCAPGly-mSc3 was a gift from Kristen Verhey (Addgene plasmid # 255801 ; http://n2t.net/addgene:255801 ; RRID:Addgene_255801)
  • For your References section:

    Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation. Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ. Elife. 2026 Feb 9;14:RP109308. doi: 10.7554/eLife.109308. 10.7554/eLife.109308 PubMed 41662400