pC1_superTagRFP-A1aY1
(Plasmid
#255800)
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Purposemammalian expression of the synthetic protein A1aY1 which recognizes the C-terminal tail of alpha-tubulin. Tagged with superTagRFP. Based on Addgene Plasmid #158754.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA1aY1
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SpeciesSynthetic
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Tag
/ Fusion Protein
- superTagRFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC1_superTagRFP-A1aY1 was a gift from Kristen Verhey (Addgene plasmid # 255800 ; http://n2t.net/addgene:255800 ; RRID:Addgene_255800) -
For your References section:
Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation. Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ. Elife. 2026 Feb 9;14:RP109308. doi: 10.7554/eLife.109308. 10.7554/eLife.109308 PubMed 41662400