pCAGGS_internal PA-TubA1A-IRES-EGFP
(Plasmid
#255804)
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Purposemammalian expression of PA-tagged human TubA1A(E254A). The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. An IRES drives expression of EGFP reporter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255804 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTubA1A
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SpeciesH. sapiens (human)
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Mutation
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Entrez GeneTUBA1A (a.k.a. B-ALPHA-1, LIS3, TUBA3)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS_internal PA-TubA1A-IRES-EGFP was a gift from Kristen Verhey (Addgene plasmid # 255804 ; http://n2t.net/addgene:255804 ; RRID:Addgene_255804) -
For your References section:
Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation. Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ. Elife. 2026 Feb 9;14:RP109308. doi: 10.7554/eLife.109308. 10.7554/eLife.109308 PubMed 41662400