LHD(B)-NovoSplit657(B)-mNeonGreen
(Plasmid
#255884)
-
PurposeExpression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mNeonGreen for fluorescence labeling, and LHD(B)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 5360
- Total vector size (bp) 6563
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNovoSplit657(B)
-
SpeciesSynthetic
- Promoter CMV
-
Tags
/ Fusion Proteins
- LHD(B) (N terminal on insert)
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.08.03.668343 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LHD(B)-NovoSplit657(B)-mNeonGreen was a gift from Julia Mahamid (Addgene plasmid # 255884 ; http://n2t.net/addgene:255884 ; RRID:Addgene_255884) -
For your References section:
De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging. Tran L, Klein S, Juergens D, Sharma S, Decarreau J, Lee GR, Wang Y, Chen W, Bera AK, Kang A, Woods J, Joyce E, Vafeados DK, Roullier N, Li X, Liu B, Bo Y, Muratspahic E, Brown TA, Grimm JB, Patel R, Lavis LD, Mahamid J, An L, Baker D. Science. 2026 Jul 16:eaeb0822. doi: 10.1126/science.aeb0822. 10.1126/science.aeb0822 PubMed 42461986
Map uploaded by the depositor.
