De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging.

Tran L, Klein S, Juergens D, Sharma S, Decarreau J, Lee GR, Wang Y, Chen W, Bera AK, Kang A, Woods J, Joyce E, Vafeados DK, Roullier N, Li X, Liu B, Bo Y, Muratspahic E, Brown TA, Grimm JB, Patel R, Lavis LD, Mahamid J, An L, Baker D
Science. 2026 Jul 16:eaeb0822. doi: 10.1126/science.aeb0822. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
255873	NovoTag494-2xFYVE	Expression plasmid for NovoTag496 fused to the 2xFYVE to localize to the endosomal membrane
255874	Tom70-NovoTag596	Expression plasmid for NovoTag596 fused to the mitochondrial localization signal Tom70
255877	H2B-NovoTag657	Expression plasmid for NovoTag657 fused to H2B to localize histones
255878	Tom70-NovoTag494(S)	Expression plasmid for NovoTag494(S), a NovoTag494 variant with short fluorescence lifetime, fused to the mitochondrial localization signal Tom70
255880	Tom70-NovoTag657(cv)	Expression plasmid for NovoTag657(cv), a NovoTag657 variant for covalent binding, fused to the mitochondrial localization signal Tom70
255883	Tom70-LHD(A)-NovoSplit657(A)-mScarlet	Expression plasmid for NovoSplit657(A), the first part of the NovoSplit system, fused to the mitochondrial localization signal Tom70, mScarlet for fluorescence labeling, and LHD(A)
255884	LHD(B)-NovoSplit657(B)-mNeonGreen	Expression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mNeonGreen for fluorescence labeling, and LHD(B)
255885	NovoSplit657(B)-mNeonGreen	Expression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mNeonGreen for fluorescence labeling.