pcDNA6-myc-BKαM-V5-His
(Plasmid #255909)

• Purpose: Express BK channel α subunit's main region (residues 44–651) as a protein module by deletion of N-terminal residues 1–43 and C-terminal residues 652–1113.
• Depositing Lab: Jiusheng Yan
• Publication: Chen et al Elife. 2026 Mar 5;14:RP107681. doi: 10.7554/eLife.107681.

Backbone

• Vector backbone: pcDNA6
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KCNMA1 (partial)
• Alt name: BK channel
• Species: H. sapiens (human)
• Insert Size (bp): 3453
• Mutation: residues 44–651 only
• GenBank ID: U11058 AAB65837
• Entrez Gene: KCNMA1 (a.k.a. BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry