Concatenated modular BK channel constructs reveal divergent stoichiometry in gating control by LRRC26 (gamma1), pore, and selectivity filter.

Chen G, Li Q, Shah K, Yan J
Elife. 2026 Mar 5;14:RP107681. doi: 10.7554/eLife.107681. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
255909	pcDNA6-myc-BKαM-V5-His	Express BK channel α subunit's main region (residues 44–651) as a protein module by deletion of N-terminal residues 1–43 and C-terminal residues 652–1113.
255910	pcDNA6-myc-BKαΔM-GFP-V5-His	Complementary plasmid for co-expression with BKαΔM module to form functional BK channels. BKα residues 94–651 are replaced by a flexible peptide linker and GFP is tagged at C-terminus.
255912	pcDNA6-BKαM3αM4-V5-His	BKαM dual-repeat expressing plasmid in which αM3 (residues 44–649) is preceded by an N-terminal sequence (MGAAAA) containing a NotI site and linked to αM4 (residues 43–649) via a flexible linker.