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Concatenated modular BK channel constructs reveal divergent stoichiometry in gating control by LRRC26 (gamma1), pore, and selectivity filter.

Chen G, Li Q, Shah K, Yan J
Elife. 2026 Mar 5;14:RP107681. doi: 10.7554/eLife.107681. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
255909pcDNA6-myc-BKαM-V5-HisExpress BK channel α subunit's main region (residues 44–651) as a protein module by deletion of N-terminal residues 1–43 and C-terminal residues 652–1113.
255910pcDNA6-myc-BKαΔM-GFP-V5-HisComplementary plasmid for co-expression with BKαΔM module to form functional BK channels. BKα residues 94–651 are replaced by a flexible peptide linker and GFP is tagged at C-terminus.

Antibodies from Article