Concatenated modular BK channel constructs reveal divergent stoichiometry in gating control by LRRC26 (gamma1), pore, and selectivity filter.
Chen G, Li Q, Shah K, Yan J
Elife. 2026 Mar 5;14:RP107681. doi: 10.7554/eLife.107681.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255909 | pcDNA6-myc-BKαM-V5-His | Express BK channel α subunit's main region (residues 44–651) as a protein module by deletion of N-terminal residues 1–43 and C-terminal residues 652–1113. |
| 255910 | pcDNA6-myc-BKαΔM-GFP-V5-His | Complementary plasmid for co-expression with BKαΔM module to form functional BK channels. BKα residues 94–651 are replaced by a flexible peptide linker and GFP is tagged at C-terminus. |