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pcDNA6-BKαM3αM4-V5-His
(Plasmid #255912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255912 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA6
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KCNMA1 (partial)
  • Alt name
    BK channel
  • Species
    H. sapiens (human)
  • Mutation
    contains αM3 (residues 44–649) preceded by an N-terminal sequence (MGAAAA) linked to αM4 (residues 43–649) via a flexible linker
  • Entrez Gene
    KCNMA1 (a.k.a. BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1)
  • Tag / Fusion Protein
    • V5-6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6-BKαM3αM4-V5-His was a gift from Jiusheng Yan (Addgene plasmid # 255912 ; http://n2t.net/addgene:255912 ; RRID:Addgene_255912)

  • For your References section:

    Concatenated modular BK channel constructs reveal divergent stoichiometry in gating control by LRRC26 (gamma1), pore, and selectivity filter. Chen G, Li Q, Shah K, Yan J. Elife. 2026 Mar 5;14:RP107681. doi: 10.7554/eLife.107681. 10.7554/eLife.107681 PubMed 41784352
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