mCherry-GEF-H1
(Plasmid
#255929)
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Purposefor use with optoTAT system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepmCherry-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-EcoRI-GEF-H1-Stop-KpnI
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-GEF-H1 was a gift from Takanari Inoue (Addgene plasmid # 255929 ; http://n2t.net/addgene:255929 ; RRID:Addgene_255929) -
For your References section:
OptoTAT reveals microtubule acetylation as a rapid trigger for GEF-H1-mediated cell migration. Deb Roy A, Gonzalez CS, Stanislauskas M, Shahid F, Yadav E, Rezek J, Inoue T. J Cell Biol. 2026 May 4;225(5):e202508095. doi: 10.1083/jcb.202508095. Epub 2026 Mar 19. 10.1083/jcb.202508095 PubMed 41854498