pHW2132
(Plasmid
#255971)
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PurposeN-terminal TAG-IN donor plasmid for fusion tagging with 3X FLAG (selection marker, SEC flanked by Lox511I)
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in United States of America -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepFH173
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRT-SEC(pDD364)-3 X FLAG-N' linker-SD-rFRT3
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SpeciesC. elegans (nematode), Synthetic
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHW2132 was a gift from Han Wang (Addgene plasmid # 255971 ; http://n2t.net/addgene:255971 ; RRID:Addgene_255971)
