Han Wang Lab: Wang Lab TAG-IN plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
255933	pHW2023	C-terminal TAG-IN donor plasmid for co-expression tagging with cGAL = cGAL(DBD)-L-QFAD (selection marker, SEC flanked by LoxP511I)
255934	pHW2072	C-terminal TAG-IN donor plasmid for co-expression tagging with TetON = rTetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
255935	pHW2073	C-terminal TAG-IN donor plasmid for co-expression tagging with TetOFF = TetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
255936	pHW2074	C-terminal TAG-IN donor plasmid for co-expression tagging with LexA = LexA(DBD)-L-QFAD (selection marker, SEC flanked by LoxP511I)
255937	pHW2075	C-terminal TAG-IN donor plasmid for co-expression tagging with QF (selection marker, SEC flanked by LoxP511I)
255938	pHW2076	C-terminal TAG-IN donor plasmid for co-expression tagging with QF2 (selection marker, SEC flanked by LoxP511I)
255939	pHW2024	C-terminal TAG-IN donor plasmid for fusion tagging with GFP-C1 (selection marker, SEC flanked by LoxP)
255940	pHW2025	C-terminal TAG-IN donor plasmid for fusion tagging with mCherry (selection marker, SEC flanked by Lox2272)
255941	pHW2077	C-terminal TAG-IN donor plasmid for fusion tagging with mNeonGreen (selection marker, SEC flanked by LoxP)
255942	pHW2126	C-terminal TAG-IN donor plasmid for fusion tagging with mScarlet-I-C1 (selection marker, SEC flanked by Lox2272)
255943	pHW2078	C-terminal TAG-IN donor plasmid for fusion tagging with TagBFP-C1 (selection marker, SEC flanked by Lox511I)
255944	pHW2079	C-terminal TAG-IN donor plasmid for fusion tagging with GFP11 7X (selection marker, SEC flanked by LoxP)
255945	pHW2080	C-terminal TAG-IN donor plasmid for fusion tagging with mIAA7::eGFP (selection marker, SEC flanked by LoxP)
255946	pHW2081	C-terminal TAG-IN donor plasmid for fusion tagging with mIAA7::mCherry (selection marker, SEC flanked by Lox2272)
255947	pHW2082	C-terminal TAG-IN donor plasmid for fusion tagging with AID (selection marker, SEC flanked by Lox511I)
255948	pHW2127	C-terminal TAG-IN donor plasmid for fusion tagging with ZF1-GFP (selection marker, SEC flanked by LoxP)
255949	pHW2083	C-terminal TAG-IN donor plasmid for fusion tagging with 3X HA (selection marker, SEC flanked by Lox511I)
255950	pHW2084	C-terminal TAG-IN donor plasmid for fusion tagging with 3X FLAG (selection marker, SEC flanked by Lox511I)
255951	pHW2085	C-terminal TAG-IN donor plasmid for fusion tagging with 3X HA turboID (selection marker, SEC flanked by Lox511I)
255952	pHW2086	C-terminal TAG-IN donor plasmid for fusion tagging with Dendra2 (selection marker, SEC flanked by Lox511I)
255953	pHW2087	C-terminal TAG-IN donor plasmid for fusion tagging with KillerRed (selection marker, SEC flanked by Lox511I)
255954	pHW2115	N-terminal TAG-IN donor plasmid for co-expression tagging with cGAL = cGAL(DBD)-L-QFAD (selection marker, SEC flanked by LoxP511I)
255955	pHW2128	N-terminal TAG-IN donor plasmid for co-expression tagging with TetON = rTetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
255956	pHW2116	N-terminal TAG-IN donor plasmid for co-expression tagging with TetOFF = TetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
255957	pHW2117	N-terminal TAG-IN donor plasmid for co-expression tagging with LexA = LexA(DBD)-L-QFAD (selection marker, SEC flanked by LoxP511I)
255958	pHW2118	N-terminal TAG-IN donor plasmid for co-expression tagging with QF (selection marker, SEC flanked by LoxP511I)
255959	pHW2143	N-terminal TAG-IN donor plasmid for co-expression tagging with QF2 (selection marker, SEC flanked by LoxP511I)
255960	pHW2119	N-terminal TAG-IN donor plasmid for fusion tagging with GFP-C1 (selection marker, SEC flanked by LoxP)
255961	pHW2159	N-terminal TAG-IN donor plasmid for fusion tagging with mCherry (selection marker, SEC flanked by Lox2272)
255962	pHW2144	N-terminal TAG-IN donor plasmid for fusion tagging with mNeonGreen (selection marker, SEC flanked by LoxP)
255963	pHW2120	N-terminal TAG-IN donor plasmid for fusion tagging with mScarlet-I-C1 (selection marker, SEC flanked by Lox2272)
255964	pHW2145	N-terminal TAG-IN donor plasmid for fusion tagging with TagBFP-C1 (selection marker, SEC flanked by Lox511I)
255965	pHW2129	N-terminal TAG-IN donor plasmid for fusion tagging with GFP11 7X (selection marker, SEC flanked by LoxP)
255966	pHW2146	N-terminal TAG-IN donor plasmid for fusion tagging with mIAA7::eGFP (selection marker, SEC flanked by LoxP)
255967	pHW2147	N-terminal TAG-IN donor plasmid for fusion tagging with mIAA7::mCherry (selection marker, SEC flanked by Lox2272)
255968	pHW2130	N-terminal TAG-IN donor plasmid for fusion tagging with AID (selection marker, SEC flanked by Lox511I)
255969	pHW2157	N-terminal TAG-IN donor plasmid for fusion tagging with ZF1-GFP (selection marker, SEC flanked by LoxP)
255970	pHW2131	N-terminal TAG-IN donor plasmid for fusion tagging with 3X HA (selection marker, SEC flanked by Lox511I)
255971	pHW2132	N-terminal TAG-IN donor plasmid for fusion tagging with 3X FLAG (selection marker, SEC flanked by Lox511I)
255972	pHW2133	N-terminal TAG-IN donor plasmid for fusion tagging with 3X HA turboID (selection marker, SEC flanked by Lox511I)
255973	pHW2148	N-terminal TAG-IN donor plasmid for fusion tagging with Dendra2 (selection marker, SEC flanked by Lox511I)
255974	pHW2149	N-terminal TAG-IN donor plasmid for fusion tagging with KillerRed (selection marker, SEC flanked by Lox511I)
255975	pHW2164	C-terminal TAG-IN destination vector with SEC-LoxP for fusion tagging (slot 5), building TAG-IN donor plasmids via Gibson cloning, or cloning in a tag by SapTrap with a tag plasmid in slot 5.
255976	pHW2165	C-terminal TAG-IN destination vector with SEC-Lox2272 for fusion tagging (slot 5), building TAG-IN donor plasmids via Gibson cloning, or cloning in a tag by SapTrap with a tag plasmid in slot 5.
255977	pHW2166	C-terminal TAG-IN destination vector with SEC-Lox511I for fusion tagging (slot 5), building TAG-IN donor plasmids via Gibson cloning, or cloning in a tag by SapTrap with a tag plasmid in slot 5.
255978	pHW2167	C-terminal TAG-IN destination vector with SEC-LoxP for co-expession tagging (slot 5), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 5.
255979	pHW2168	C-terminal TAG-IN destination vector with SEC-Lox2272 for co-expession tagging (slot 5), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 5
255980	pHW2169	C-terminal TAG-IN destination vector with SEC-Lox511I for co-expession tagging (slot 5), building TAG-IN donor plasmids via Gibson cloning, or cloning in a tag by SapTrap with a tag plasmid in slot 5.
255981	pHW2173	N-terminal TAG-IN destination vector with SEC-LoxP for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
255982	pHW2174	N-terminal TAG-IN destination vector with SEC-Lox2272 for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
255983	pHW2175	N-terminal TAG-IN destination vector with SEC-Lox511I for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
255984	pHW2176	N-terminal TAG-IN destination vector with SEC-LoxP co-expression tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
255985	pHW2177	N-terminal TAG-IN destination vector with SEC-Lox2272 co-expression tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
255986	pHW2178	N-terminal TAG-IN destination vector with SEC-Lox511I co-expression tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.