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pHW2177
(Plasmid #255985)

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  • Purpose
    N-terminal TAG-IN destination vector with SEC-Lox2272 co-expression tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
  • Depositing Lab
    Han Wang
  • Depositing Organization
    University of Wisconsin, Madison
    Located in United States of America
  • Publication
    Wang Lab TAG-IN plasmids (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255985 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFH174
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAG-SEC(pDD382 with Lox2272)-GGT-rSapI-SpeI-SapI-ACG-SL2
  • Species
    C. elegans (nematode), Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHW2177 was a gift from Han Wang (Addgene plasmid # 255985 ; http://n2t.net/addgene:255985 ; RRID:Addgene_255985)
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