PMXS-OST4-mScarlet-3xMYC-IRES-blasticidin
(Plasmid
#255992)
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PurposePMXS construct expressing OST4-mScarlet-3xMYC with an IRES-blasticidin cassette for use as an IP control.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMXS-IRES-Blast
- Backbone size w/o insert (bp) 5639
- Total vector size (bp) 6587
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOST4-mScarlet-3xMYC
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SpeciesH. sapiens (human)
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Insert Size (bp)961
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MutationN/A
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Entrez GeneOST4
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Tag
/ Fusion Protein
- mScarlet-3xMYC (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CAAAGTAGACGGCATCGCA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PMXS-OST4-mScarlet-3xMYC-IRES-blasticidin was a gift from Kivanc Birsoy (Addgene plasmid # 255992 ; http://n2t.net/addgene:255992 ; RRID:Addgene_255992) -
For your References section:
SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis. Liu S, Gad M, Li C, Cho K, Liu Y, Wangdu K, Belay V, Millet A, Kojima H, Sanford H, Wolk M, Urnavicius L, Fedorova M, Patti GJ, Vinogradova EV, Hite RK, Birsoy K. Nat Cell Biol. 2026 Apr 17. doi: 10.1038/s41556-026-01922-y. 10.1038/s41556-026-01922-y PubMed 41998286