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SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis.

Liu S, Gad M, Li C, Cho K, Liu Y, Wangdu K, Belay V, Millet A, Kojima H, Sanford H, Wolk M, Urnavicius L, Fedorova M, Patti GJ, Vinogradova EV, Hite RK, Birsoy K
Nat Cell Biol. 2026 Apr 17. doi: 10.1038/s41556-026-01922-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
255987PMXS-EMC3-mScarlet-3xHA-IRES-bleomycinPMXS construct expressing EMC3-mScarlet-3xHA with an IRES-bleomycin cassette for ER-IP
255988PMXS-EMC3-mScarlet-3xMYC-IRES-bleomycinPMXS construct expressing EMC3-mScarlet-3xMYC with an IRES-bleomycin cassette for use as an IP control
255989PMXS-EMC3-mScarlet-3xHA-IRES-blasticidinPMXS construct expressing EMC3-mScarlet-3xHA with an IRES-blasticidin cassette for ER-IP
255990PMXS-EMC3-mScarlet-3xMYC-IRES-blasticidinPMXS construct expressing EMC3-mScarlet-3xMYC with an IRES-blasticidin cassette for use as an IP control.
255991PMXS-OST4-mScarlet-3xHA-IRES-blasticidinPMXS construct expressing OST4-mScarlet-3xHA with an IRES-blasticidin cassette for ER-IP
255992PMXS-OST4-mScarlet-3xMYC-IRES-blasticidinPMXS construct expressing OST4-mScarlet-3xMYC with an IRES-blasticidin cassette for use as an IP control.
257599pMXS_IRES_GFP_FLAG-33A1Expression of FLAG-tagged human SLC33A1 with GFP reporter
257600pLJC6-FLAG-SLC33A1-BlastLentiviral expression of FLAG-tagged human SLC33A1
257601pLJC6-FLAG-SLC33A1-Y225A-BlastExpression of FLAG-tagged human SLC33A1 Y225A mutant
257602pLJC6-FLAG-SLC33A1-N229A-BlastExpression of FLAG-tagged human SLC33A1 N229A mutant
257603pLJC6-FLAG-SLC33A1-Q356A-BlastExpression of FLAG-tagged human SLC33A1 Q356A mutant
257604pLJC6-FLAG-SLC33A1-Y390A-BlastExpression of FLAG-tagged human SLC33A1 Y390A mutant
257605pLJC6-FLAG-SLC33A1-Y418A-BlastExpression of FLAG-tagged human SLC33A1 Y418A mutant
257606pLJC6-FLAG-SLC33A1-Q422A-BlastExpression of FLAG-tagged human SLC33A1 Q422A mutant
257607PCW57.1-ER-GshF-GFPDoxycycline-inducible expression of ER-targeted GshF-GFP
257609lentiCRISPR-v2_sg_SLC33A1CRISPR knockout of human SLC33A1
257610lentiCRISPR-v2_sg_Slc33a1CRISPR knockout of mouse Slc33a1
257611lentiCRISPR-v2_sg2_SYVN1CRISPR knockout of SYVN1 using sgRNA 2
257612lentiCRISPR-v2_sg4_SYVN1CRISPR knockout of SYVN1 using sgRNA 4
257613lentiCRISPR-v2_sg1_Ero1aCRISPR knockout of Ero1a using sgRNA 1
257614lentiCRISPR-v2_sg2_Ero1aCRISPR knockout of Ero1a using sgRNA 2

Antibodies from Article