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pFLAG-CMV2-mMLL4fusion
(Plasmid #256014)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256014 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFLAG-CMV-2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mMLL4 fusion
  • Alt name
    KMT2D
  • Species
    H. sapiens (human)
  • Mutation
    PHD(PHD4-6) and SET(Catalytic domain) along with mutation in PHD4-6(E1516A/E1517A/D1518A/E1544A)
  • Entrez Gene
    KMT2D (a.k.a. AAD10, ALR, BCAHH, CAGL114, KABUK1, KMS, MLL2, MLL4, TNRC21)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Lee Lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-CMV2-mMLL4fusion was a gift from Min Gyu Lee (Addgene plasmid # 256014 ; http://n2t.net/addgene:256014 ; RRID:Addgene_256014)

  • For your References section:

    Trans-tail regulation of MLL4-catalyzed H3K4 methylation by H4R3 symmetric dimethylation is mediated by a tandem PHD of MLL4. Dhar SS, Lee SH, Kan PY, Voigt P, Ma L, Shi X, Reinberg D, Lee MG. Genes Dev. 2012 Dec 15;26(24):2749-62. doi: 10.1101/gad.203356.112. 10.1101/gad.203356.112 PubMed 23249737
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