pAAV-CMV-caAkt-P2A-AktKiMBI
(Plasmid
#256282)
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PurposeExpresses Akt KiMBI to report Akt inhibition with bioluminescence, with caAkt to increase basal Akt activity, and can be packaged into AAV particles
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecaAkt-P2A-AktKiMBI
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CMV-caAkt-P2A-AktKiMBI was a gift from Michael Lin (Addgene plasmid # 256282 ; http://n2t.net/addgene:256282 ; RRID:Addgene_256282) -
For your References section:
Pharmacodynamics of Akt drugs revealed by a kinase-modulated bioluminescent indicator. Wu Y, Hao C, Gao C, Hageman M, Lee S, Kirkland TA, Gray NS, Su Y, Lin MZ. Nat Chem Biol. 2025 Aug;21(8):1194-1204. doi: 10.1038/s41589-025-01846-y. Epub 2025 Feb 11. 10.1038/s41589-025-01846-y PubMed 39934397
