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pAAV-CMV-caAkt-P2A-AktKiMBImut
(Plasmid #256283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256283 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    caAkt-P2A-AktKiMBImut
  • Species
    H. sapiens (human)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-caAkt-P2A-AktKiMBImut was a gift from Michael Lin (Addgene plasmid # 256283 ; http://n2t.net/addgene:256283 ; RRID:Addgene_256283)

  • For your References section:

    Pharmacodynamics of Akt drugs revealed by a kinase-modulated bioluminescent indicator. Wu Y, Hao C, Gao C, Hageman M, Lee S, Kirkland TA, Gray NS, Su Y, Lin MZ. Nat Chem Biol. 2025 Aug;21(8):1194-1204. doi: 10.1038/s41589-025-01846-y. Epub 2025 Feb 11. 10.1038/s41589-025-01846-y PubMed 39934397
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