pIRES2-AcGFP1-PX-Gal-3
(Plasmid
#256298)
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PurposeExpresses PX-Gal-3 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepIRES2-AcGFP1
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 6824
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePX-Gal-3
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SpeciesH. sapiens (human)
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Entrez GeneLGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypurchased from Genscript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES2-AcGFP1-PX-Gal-3 was a gift from Mia Huang (Addgene plasmid # 256298 ; http://n2t.net/addgene:256298 ; RRID:Addgene_256298) -
For your References section:
Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling. Joeh E, O'Leary T, Li W, Hawkins R, Hung JR, Parker CG, Huang ML. Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27329-27338. doi: 10.1073/pnas.2009206117. Epub 2020 Oct 16. 10.1073/pnas.2009206117 PubMed 33067390