Skip to main content
Addgene
Open Menu

pBK proK-lacI MaPylT metG MMIF
(Plasmid #256314)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256314 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    ColE1
  • Total vector size (bp) 4596
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Supplement with 1% glucose to suppress basal tRNA expression and mitigate toxicity
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    M. alvus pyrrolysyl synthetase-MMIF
  • Insert Size (bp)
    828
  • Mutation
    V205I, Y206F
  • Promoter metG

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tRNA-MaPyl
  • Insert Size (bp)
    71
  • Promoter proK-lacI

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBK proK-lacI MaPylT metG MMIF was a gift from Abhishek Chatterjee (Addgene plasmid # 256314 ; http://n2t.net/addgene:256314 ; RRID:Addgene_256314)

  • For your References section:

    Co-Translational Incorporation of (R)- and (S)-beta(2)-Hydroxyacids In Vivo: Directed Evolution of Efficient Aminoacyl-tRNA Synthetases. Soni C, Pressimone M, Nair M, Szalay K, Hall M, Hamlish N, Solivan AC, Schepartz A, Chatterjee A. J Am Chem Soc. 2026 Mar 11;148(9):9382-9392. doi: 10.1021/jacs.5c18595. Epub 2026 Feb 27. 10.1021/jacs.5c18595 PubMed 41757713
Related items: