Co-Translational Incorporation of (R)- and (S)-beta(2)-Hydroxyacids In Vivo: Directed Evolution of Efficient Aminoacyl-tRNA Synthetases.

Soni C, Pressimone M, Nair M, Szalay K, Hall M, Hamlish N, Solivan AC, Schepartz A, Chatterjee A
J Am Chem Soc. 2026 Mar 11;148(9):9382-9392. doi: 10.1021/jacs.5c18595. Epub 2026 Feb 27. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
256312	pBK proK-lacI MaPylT metG MaPylRS	Plasmid encoding M. alvus pyrrolysyl synthetase and inducible tRNA
256313	pBK proK-lacI MaPylT metG FCVF	Plasmid encoding mutant M. alvus pyrrolysyl synthetase-FCVF and inducible tRNA for β2-hydroxy acid monomers
256314	pBK proK-lacI MaPylT metG MMIF	Plasmid encoding mutant M. alvus pyrrolysyl synthetase-MMIF and inducible tRNA for β2-hydroxy acid, β2-amino acid and malonic acid monomers
256315	pEvol T5-lac Nluc WT	Plasmid encoding reporter gene Nanoluciferase wild-type
256316	pEvol T5-lac Nluc-3-TAG	Plasmid encoding Nanoluciferase with a premature TAG stop codon at 3rd position
256317	pET22b 10xHis-C-term-MaPylRS	Plasmid encoding M. alvus pyrrolysyl synthetase with a C-terminal 10xHis tag
256319	pET22b 10xHis-C-term-MaPylRS-MMIF	Plasmid encoding mutant M. alvus pyrrolysyl synthetase-FCVF with a C-terminal 10xHis tag
256353	pB1-MaPylRS-MMIF-4xG55T-TAG	Plasmid encoding M. alvus pyrrolysyl synthetase-MMIF and tRNA for β2-hydroxy acid monomers
256354	pB1-EGFP-12xHis	Plasmid encoding EGFP reporter with His tag for HEK cell expression
256355	pB1-EGFP-39-TAG-12xHis	Plasmid encoding EGFP-39-TAG reporter with His tag for HEK cell expression