Skip to main content
Addgene
Open Menu

sgOPTI:U6-gRNA-CS1-puroR
(Plasmid #256351)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256351 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    sgOpti
  • Backbone size w/o insert (bp) 9188
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA
  • Alt name
    single guide RNA
  • gRNA/shRNA sequence
    N/A
  • Promoter U6 promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.16.676677 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgOPTI:U6-gRNA-CS1-puroR was a gift from Jesse Engreitz (Addgene plasmid # 256351 ; http://n2t.net/addgene:256351 ; RRID:Addgene_256351)

  • For your References section:

    An unbiased survey of distal element-gene regulatory interactions with direct-capture targeted Perturb-seq. Ray J, Jagoda E, Sheth MU, Galante J, Amgalan D, Gschwind AR, Munger CJ, Huang J, Munson G, Murphy M, Mattei E, Barry T, Singh V, Baskaran A, Kang H, Katsevich E, Steinmetz LM, Engreitz JM. bioRxiv [Preprint]. 2025 Sep 17:2025.09.16.676677. doi: 10.1101/2025.09.16.676677. 10.1101/2025.09.16.676677 PubMed 41000760
Related items: