An unbiased survey of distal element-gene regulatory interactions with direct-capture targeted Perturb-seq.
Ray J, Jagoda E, Sheth MU, Galante J, Amgalan D, Gschwind AR, Munger CJ, Huang J, Munson G, Murphy M, Mattei E, Barry T, Singh V, Baskaran A, Kang H, Katsevich E, Steinmetz LM, Engreitz JM
bioRxiv [Preprint]. 2025 Sep 17:2025.09.16.676677. doi: 10.1101/2025.09.16.676677.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256351 | sgOPTI:U6-gRNA-CS1-puroR | lenti virus vector expressing a Cas9 guide RNA under a human U6 promoter. Contains capture sequence 1 (CS1) in the Cas9 scaffold. Contains puromycin resistance cassette. |