|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25636||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro
- Backbone size w/o insert (bp) 7032
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)42
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Cloning method Restriction Enzyme
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO-p53-shRNA-427 was a gift from Todd Waldman (Addgene plasmid # 25636 ; http://n2t.net/addgene:25636 ; RRID:Addgene_25636)
For your References section:Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA. Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T. Mol Cell Biol. 2007 Jan . 27(2):662-77. 10.1128/MCB.00537-06 PubMed 17060456