Sc Pol1(STS)-pRS406-kanMX
(Plasmid
#256369)
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PurposeRecombine a mutated Sc Pol1 gene [Pol1(STS)] into a diploid strain of Saccharomyces cerevisiae containing a kanamex cassette in place of one of its native Pol1 genes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRS406
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Vector typeYeast Expression ; S. cerevisiae recombination; NOT for over-expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSc Pol1(STS)
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SpeciesS. cerevisiae (budding yeast)
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MutationD996S; D998S (=STS)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sc Pol1(STS)-pRS406-kanMX was a gift from Michael O'Donnell (Addgene plasmid # 256369 ; http://n2t.net/addgene:256369 ; RRID:Addgene_256369)