Michael O'Donnell Lab: O'Donnell Lab Plasmids Apr 2026
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256368 | pJF3KB1 | Template for single molecule work. Linearized, nicked, & put in an optical trap, can create a long dsDNA template with ~3kb ssDNA region in the middle. |
| 256369 | Sc Pol1(STS)-pRS406-kanMX | Recombine a mutated Sc Pol1 gene [Pol1(STS)] into a diploid strain of Saccharomyces cerevisiae containing a kanamex cassette in place of one of its native Pol1 genes. |
| 256370 | pRS406-kanMX | Insert a gene to create a plasmid that can be used to recombine into a diploid strain of Saccharomyces cerevisiae containing a kanamex cassette in place of one copy of the gene being targetted. |
| 256371 | Sc Pol1-pRS406-kanMX | Recombine a wild-type Sc Pol1 gene into a diploid strain of Saccharomyces cerevisiae containing a kanamex cassette in place of one of its native Pol1 genes. |
| 256372 | Sc FLAG-Elg1-pRSFDuet | over-express Sc Elg1 gene in E. coli with an N-terminal 3X FLAG tag. |