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pJF3KB1
(Plasmid #256368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256368 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRGEB32 (Addgene #63142)
  • Backbone manufacturer
    Yinong Yang
  • Vector type
    Bacterial Expression ; Not for over-expression; for structural use in single-molecule studies.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ~3kb AT-rich region fronm pLW58
  • Species
    Unspecified
  • Insert Size (bp)
    3000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)
  • 3′ cloning site Unspecified (unknown if destroyed)
  • 5′ sequencing primer Unspecified
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid containing an AT-rich 3kb region flanked by Nb.BvcCI nicking sites to generate ssDNA gaps for single-molecule studies, Linearized with Bsa I & annealed to biotinylated oligos to allow attachment to beads on both ends.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJF3KB1 was a gift from Michael O'Donnell (Addgene plasmid # 256368 ; http://n2t.net/addgene:256368 ; RRID:Addgene_256368)
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