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MCU-mGFP-EMRE-TwinStrep pTRE bidirectional
(Plasmid #256374)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256374 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTRE bidirctional
  • Backbone size w/o insert (bp) 7343
  • Vector type
    Mammalian Expression ; PiggyBac
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    MCU
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2004
  • Entrez Gene
    MCU (a.k.a. C10orf42, CCDC109A, HsMCU)
  • Promoter TRE3G BI
  • Tag / Fusion Protein
    • mGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    EMRE/SMDT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    414
  • Entrez Gene
    SMDT1 (a.k.a. C22orf32, DDDD, EMRE)
  • Promoter TRE3G BI
  • Tag / Fusion Protein
    • TwinStrep (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCU-mGFP-EMRE-TwinStrep pTRE bidirectional was a gift from Dipayan Chaudhuri (Addgene plasmid # 256374 ; http://n2t.net/addgene:256374 ; RRID:Addgene_256374)

  • For your References section:

    Matrix polyamines regulate bidirectional calcium flux through MCU. Wang Q, Balderas E, Ramos AJ, Nwafor A, McManus JS, Rai NK, Masini YB, Eberhardt DR, Rekate EC, Balynas AM, Yin X, Stewart TM, Cao E, Stary-Weinzinger A, Chaudhuri D. Structure. 2026 Jul 27:S0969-2126(26)00212-1. doi: 10.1016/j.str.2026.07.002. 10.1016/j.str.2026.07.002 PubMed 42508402
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