pSNG-S
(Plasmid
#256383)
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Purpose(Empty Backbone) An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal SBP-HA-S tag and a C-terminal 3xFLAG tag.
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Depositing Lab
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Depositing OrganizationCentre for Cellular and Molecular Biology (CSIR-CCMB)
Located in India -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepSNG-S
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- SBP-HA-S (N terminal on backbone)
- 3xFLAG (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- SBP-HA-S (N terminal on backbone)
- 3xFLAG (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSNG-S was a gift from Vinay Nandicoori (Addgene plasmid # 256383 ; http://n2t.net/addgene:256383 ; RRID:Addgene_256383) -
For your References section:
Modular toolkit to facilitate molecular manipulations in mycobacteria. Khuntia AK, Dabla A, Kapoor Y, Chakraborty A, Arputharaj PE, Nandicoori VK. J Bacteriol. 2026 Apr 23;208(4):e0047425. doi: 10.1128/jb.00474-25. Epub 2026 Mar 16. 10.1128/jb.00474-25 PubMed 41837628
