Modular toolkit to facilitate molecular manipulations in mycobacteria.
Khuntia AK, Dabla A, Kapoor Y, Chakraborty A, Arputharaj PE, Nandicoori VK
J Bacteriol. 2026 Apr 23;208(4):e0047425. doi: 10.1128/jb.00474-25. Epub 2026 Mar 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256383 | pSNG-S | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal SBP-HA-S tag and a C-terminal 3xFLAG tag. |
| 256384 | pSNG-F | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains a 3xFLAG tag at both N- and C- terminus |
| 256386 | pSNG-mC | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal Red fluorescent protein (mCherry) tag and a C-terminal 3xFLAG tag. |
| 256388 | pSCG-F | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains a C-terminal 3xFLAG tag |
| 256391 | pSNM-S | An E. coli-Mycobacterium shuttle plasmid designed for integration at the MS6 attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal SBP-HA-S tag and a C-terminal 3xFLAG tag. |
| 256392 | pSNM-F | An E. coli-Mycobacterium shuttle plasmid designed for integration at the MS6 attB site to enable stable gene expression in Mycobacterium species. It contains a 3xFLAG tag at both N- and C- terminus |
| 256394 | pSNM-mC | An E. coli-Mycobacterium shuttle plasmid designed for integration at the MS6 attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal Red fluorescent protein (mCherry) tag and a C-terminal 3xFLAG tag. |
| 256396 | pSCM-F | An E. coli-Mycobacterium shuttle plasmid designed for integration at the MS6 attB site to enable stable gene expression in Mycobacterium species. It contains a C-terminal 3xFLAG tag |
| 256397 | pSCM-GFP | An E. coli-Mycobacterium shuttle plasmid designed for integration at the MS6 attB site to enable stable gene expression in Mycobacterium species. It contains a C-terminal Green fluorescent protein (GFP) tag. |
| 256398 | pSCM-mC | An E. coli-Mycobacterium shuttle plasmid designed for integration at the MS6 attB site to enable stable gene expression in Mycobacterium species. It contains a C-terminal Red fluorescent protein (mCherry) tag. |
| 256399 | pSNW-S | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Tweety attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal SBP-HA-S tag and a C-terminal 3xFLAG tag. |
| 256400 | pSNW-F | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Tweety attB site to enable stable gene expression in Mycobacterium species. It contains a 3xFLAG tag at both N- and C- terminus |
| 256401 | pSNW-GFP | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Tweety attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal Green fluorescent protein (GFP) tag and a C-terminal 3xFLAG tag. |
| 256408 | pSNE-F | An E. coli-Mycobacterium shuttle plasmid designed for extrachromosomal overexpression in mycobacterial species. It contains a 3xFLAG tag at both N- and C- terminus |
| 256409 | pSNE-GFP | An E. coli-Mycobacterium shuttle plasmid designed for extrachromosomal overexpression in mycobacterial species. It contains an N-terminal Green fluorescent protein (GFP) tag and a C-terminal 3xFLAG tag. |
| 256410 | pSNE-mC | An E. coli-Mycobacterium shuttle plasmid designed for extrachromosomal overexpression in mycobacterial species. It contains an N-terminal Red fluorescent protein (mCherry) tag and a C-terminal 3xFLAG tag. |
| 256412 | pSCE-F | An E. coli-Mycobacterium shuttle plasmid designed for extrachromosomal overexpression in mycobacterial species. It contains a C-terminal 3xFLAG tag |
| 256413 | pSCE-GFP | An E. coli-Mycobacterium shuttle plasmid designed for extrachromosomal overexpression in mycobacterial species. It contains a C-terminal Green fluorescent protein (GFP) tag. |
| 256414 | pSCE-mC | An E. coli-Mycobacterium shuttle plasmid designed for extrachromosomal overexpression in mycobacterial species. It contains a C-terminal Red fluorescent protein (mCherry) tag. |