Modular toolkit to facilitate molecular manipulations in mycobacteria.
Khuntia AK, Dabla A, Kapoor Y, Chakraborty A, Arputharaj PE, Nandicoori VK
J Bacteriol. 2026 Apr 23;208(4):e0047425. doi: 10.1128/jb.00474-25. Epub 2026 Mar 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256383 | pSNG-S | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal SBP-HA-S tag and a C-terminal 3xFLAG tag. |
| 256386 | pSNG-mC | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains an N-terminal Red fluorescent protein (mCherry) tag and a C-terminal 3xFLAG tag. |
| 256388 | pSCG-F | An E. coli-Mycobacterium shuttle plasmid designed for integration at the Giles attB site to enable stable gene expression in Mycobacterium species. It contains a C-terminal 3xFLAG tag |