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GB5096
(Plasmid #256561)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256561 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLX-B2_alpha2
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TMV:HmS
  • Species
    Synthetic
  • Mutation
    BsaI and BsmBI sites removed
  • Promoter P35S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.05.668616 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB5096 was a gift from Diego Orzaez (Addgene plasmid # 256561 ; http://n2t.net/addgene:256561 ; RRID:Addgene_256561)

  • For your References section:

    Bioluminescent sentinel plants enable autonomous diagnostics of viral infections. Calvache C, Rodriguez-Rodriguez M, Vazquez-Vilriales V, Garcia-Perez E, Fleiss A, Ezzeddin-Ayoub M, Pasin F, Daros JA, Sarkisyan KS, Orzaez D, Vazquez-Vilar M. Nat Commun. 2026 May 29. doi: 10.1038/s41467-026-73810-w. 10.1038/s41467-026-73810-w PubMed 42215479
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