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Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.

Calvache C, Rodriguez-Rodriguez M, Vazquez-Vilriales V, Garcia-Perez E, Fleiss A, Ezzeddin-Ayoub M, Pasin F, Daros JA, Sarkisyan KS, Orzaez D, Vazquez-Vilar M
Nat Commun. 2026 May 29. doi: 10.1038/s41467-026-73810-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
256557GB5058Transcriptional unit of Bean Yellow Dwarf Virus-based replicon expressing ASCL gene (a HispS substitute).
256558GB5059Transcriptional unit of Bean Yellow Dwarf Virus-based replicon expressing HmS gene (a HispS substitute).
256559GB5060Transcriptional unit of Bean Yellow Dwarf Virus-based replicon expressing PKS2 gene (a HispS substitute).
256560GB4901Module for the expression of the Rep/RepA proteins for BeYDV replication and the p19 silencing suppressor.
256561GB5096Transcriptional unit of Tobacco Mosaic Virus-based replicon expressing HmS gene (a HispS substitute).
256562GB5097Transcriptional unit of Tobacco Mosaic Virus-based replicon expressing ASCL gene (a HispS substitute).
256563GB5098Transcriptional unit of Tobacco Mosaic Virus-based replicon expressing PKS2 gene (a HispS substitute).
256567GB5091Module for the expression of N. nambi NPGA, H3H, HISPS, and CPH genes.
256568GB5482TU for the expression of LUZ:GS1x:DsRed under the P35S promoter and TNOS.
256569GB4918TU for the expression of DsRed:GS1X:LUZ under the P35S promoter and TNOS.
256570GB5483TU for the expression of LUZ:GS3x:DsRed under the P35S promoter and TNOS.
256571GB5484TU for the expression of LUZ:OD:DsRed under the P35S promoter and TNOS.
256573GB5486TU for the expression of LUZ:Acrlinker:DsRed under the P35S promoter and TNOS.
256575GB5488TU for the expression of LUZ:GGS:TEV:DsRed under the P35S promoter and TNOS.
256576GB5489TU for the expression of LUZ:ODTEV:DsRed under the P35S promoter and TNOS.
256577GB5490TU for the expression of LUZ:GGSTVMV:DsRed under the P35S promoter and TNOS.
256579GB5502TU for the expression of LUZ fused PdaC1 as Ntag.
256581GB5748TU for the constitutive expression of the Tobacco Vein Mottling Virus NIa protease under the P35S promoter and TNOS.
256582GB1203TU for the expression of the silencing suppressor P19 under the P35S promoter and TNOS.
256583GB1236Empty vector ( Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector).
256584GB3477TU of Luz gene from N. nambi codon optimized for N.benthamiana under the P35S promoter and TNOS.
256585GB5510Module for the expression of plXB2alpha1:HygR- Disct02-LUZ:GS1xTEV:DsRed-Disct02.
256586GB5511Module for the expression of plXB2alpha1:HygR-Disct02-LUZ:ODTEV:DsRed- Disct02.
256587GB5108Module for the expression of nptII and the N. nambi NPGA, H3H, HISPS and CPH genes flanked by insulators.
256588GB5512Module for the expression of plXB2alpha1:HygR- Disct02-LUZ:GGSTVMV:DsRed-Disct02 in PLX vector.

Antibodies from Article