GB1236
(Plasmid
#256583)
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PurposeEmpty vector ( Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector).
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Depositing Lab
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Depositing OrganizationInstituto de Biologia Molecular y Celular de Plantas
Located in Spain -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDGB3_alpha2
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEV
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SpeciesSynthetic
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MutationBsaI and BsmBI sites removed
- Promoter None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.08.05.668616 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GB1236 was a gift from Diego Orzaez (Addgene plasmid # 256583 ; http://n2t.net/addgene:256583 ; RRID:Addgene_256583) -
For your References section:
Bioluminescent sentinel plants enable autonomous diagnostics of viral infections. Calvache C, Rodriguez-Rodriguez M, Vazquez-Vilriales V, Garcia-Perez E, Fleiss A, Ezzeddin-Ayoub M, Pasin F, Daros JA, Sarkisyan KS, Orzaez D, Vazquez-Vilar M. Nat Commun. 2026 May 29. doi: 10.1038/s41467-026-73810-w. 10.1038/s41467-026-73810-w PubMed 42215479