pLKO H2BmRFP-2A-puro v4 trcr filler
(Plasmid
#256775)
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Purpose(Empty Backbone) lentiviral construct with H2BmRFP-2A-puromycin and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLKO Cre v4 trcr filler (no SapI)
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO H2BmRFP-2A-puro v4 trcr filler was a gift from Daniel Schramek (Addgene plasmid # 256775 ; http://n2t.net/addgene:256775 ; RRID:Addgene_256775)