Daniel Schramek Lab: Schramek lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256774 | pLKO Cre v4 trcr filler | lentiviral construct with Cre recombinase and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256775 | pLKO H2BmRFP-2A-puro v4 trcr filler | lentiviral construct with H2BmRFP-2A-puromycin and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256776 | pXPR502 PPH-Cre v14 trcr filler | lentiviral construct with PPH-Cre recombinase (P65-PP7 Coat Protein-HSF1-Cre) U6 driven gRNA cassette with the v14 modified trcrRNA. gRNAs can be cloned in by digesting the filler with SapI. |
| 256777 | pXPR502 PPH-puro v14 trcr filler | lentiviral construct with PPH-Puro (P65-PP7 Coat Protein-HSF1-Puro) U6 driven gRNA cassette with the v14 modified trcrRNA. gRNAs can be cloned in by digesting the filler with SapI. |
| 256778 | pXPR502 PPH-Cre dual trcrs filler | lentiviral construct with PPH-Cre recombinase (P65-PP7 Coat Protein-HSF1-Cre) U6 driven gRNA cassettes with the v4 and v14 modified trcrRNAs. gRNAs can be cloned in with SapI/BsmBI. |
| 256779 | pLV PPH-GFP | lentiviral construct with PPH-GFP (P65-PP7 Coat Protein-HSF1-GFP) |
| 256780 | pXPR502 H2BmRFP-2A-puro v14 trcr filler | lentiviral construct with H2BmRFP-2A-puromycin and U6 driven gRNA cassette with the v14 modified trcrRNA. gRNAs can be cloned in by digesting the filler with SapI. |
| 256781 | pLKO H2BmRFP-2A-PLGRKT v4 trcr filler | lentiviral construct with H2BmRFP-2A-PLGRKT (human) and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256782 | pLKO H2BmRFP-2A-Plgrkt v4 trcr filler | lentiviral construct with H2BmRFP-2A-Plgrkt (mouse) and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256783 | pLKO H2BmRFP-2A-Plgrkt K147R v4 trcr filler | lentiviral construct with H2BmRFP-2A-Plgrkt (mouse) K147R and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256784 | pLKO H2BmRFP-2A-Plgrkt Δ139-147 v4 trcr filler | lentiviral construct with H2BmRFP-2A-Plgrkt (mouse) Δ139-147 and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256785 | pLKO H2BmRFP-2A-puro HA v4 trcr filler | lentiviral construct with H2BmRFP-2A-puromycin, HA, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256786 | pLKO H2BmRFP-2A-puro Plgrkt v4 trcr filler | lentiviral construct with H2BmRFP-2A-puromycin, Plgrkt, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256787 | pLKO H2BmRFP-2A-puro Plgrkt-HA v4 trcr filler | lentiviral construct with H2BmRFP-2A-puromycin, Plgrkt-HA, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256788 | pLKO H2BmRFP-2A-puro PLGRKT v4 trcr filler | lentiviral construct with H2BmRFP-2A-puromycin, PLGRKT, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256789 | pLKO HA-2A-Cre v4 trcr filler | lentiviral construct with HA-2A-Cre recombinase and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256790 | pLKO Cre-2A-Plgrkt v4 trcr filler | lentiviral construct with Cre recombinase-2A-Plgrkt and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256791 | pLKO Cre-2A-Plgrkt K147R v4 trcr filler | lentiviral construct with Cre recombinase-2A-Plgrkt K147R and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |
| 256792 | pLKO Cre-2A-Plgrkt Δ139-147 v4 trcr filler | lentiviral construct with Cre recombinase-2A-Plgrkt Δ139-147 and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI. |