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Aneuploidy selects for the acquisition of driver genes in breast cancer.

Al-Zahrani KN, Langille ER, Nurtanto J, Obersterescu A, Teng K, Lowden C, Dessapt J, Chiu CH, Caldwell LV, Cook DP, Perez-Castro MA, Berman JM, Tsai R, Bahcheli AT, Mbamalu G, Wu S, Narimatsu M, Lopes AG, Fotiadou I, Chan K, Zhang L, Bang KWA, Parsons MJ, Mourao L, Temel EI, McCulla L, Sravya P, Zhang L, Sajjakulnukit P, Lyssiotis CA, Borowsky AD, Scheele CLGJ, Wahl DR, Jackson HW, Stewart KS, Fuchs E, Egan SE, Pujana MA, Reimand J, Wrana JL, Schramek D
Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10752-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
256774pLKO Cre v4 trcr fillerlentiviral construct with Cre recombinase and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256775pLKO H2BmRFP-2A-puro v4 trcr fillerlentiviral construct with H2BmRFP-2A-puromycin and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256776pXPR502 PPH-Cre v14 trcr fillerlentiviral construct with PPH-Cre recombinase (P65-PP7 Coat Protein-HSF1-Cre) U6 driven gRNA cassette with the v14 modified trcrRNA. gRNAs can be cloned in by digesting the filler with SapI.
256777pXPR502 PPH-puro v14 trcr fillerlentiviral construct with PPH-Puro (P65-PP7 Coat Protein-HSF1-Puro) U6 driven gRNA cassette with the v14 modified trcrRNA. gRNAs can be cloned in by digesting the filler with SapI.
256778pXPR502 PPH-Cre dual trcrs fillerlentiviral construct with PPH-Cre recombinase (P65-PP7 Coat Protein-HSF1-Cre) U6 driven gRNA cassettes with the v4 and v14 modified trcrRNAs. gRNAs can be cloned in with SapI/BsmBI.
256779pLV PPH-GFPlentiviral construct with PPH-GFP (P65-PP7 Coat Protein-HSF1-GFP)
256780pXPR502 H2BmRFP-2A-puro v14 trcr fillerlentiviral construct with H2BmRFP-2A-puromycin and U6 driven gRNA cassette with the v14 modified trcrRNA. gRNAs can be cloned in by digesting the filler with SapI.
256781pLKO H2BmRFP-2A-PLGRKT v4 trcr fillerlentiviral construct with H2BmRFP-2A-PLGRKT (human) and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256782pLKO H2BmRFP-2A-Plgrkt v4 trcr fillerlentiviral construct with H2BmRFP-2A-Plgrkt (mouse) and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256783pLKO H2BmRFP-2A-Plgrkt K147R v4 trcr fillerlentiviral construct with H2BmRFP-2A-Plgrkt (mouse) K147R and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256784pLKO H2BmRFP-2A-Plgrkt Δ139-147 v4 trcr fillerlentiviral construct with H2BmRFP-2A-Plgrkt (mouse) Δ139-147 and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256785pLKO H2BmRFP-2A-puro HA v4 trcr fillerlentiviral construct with H2BmRFP-2A-puromycin, HA, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256786pLKO H2BmRFP-2A-puro Plgrkt v4 trcr fillerlentiviral construct with H2BmRFP-2A-puromycin, Plgrkt, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256787pLKO H2BmRFP-2A-puro Plgrkt-HA v4 trcr fillerlentiviral construct with H2BmRFP-2A-puromycin, Plgrkt-HA, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256788pLKO H2BmRFP-2A-puro PLGRKT v4 trcr fillerlentiviral construct with H2BmRFP-2A-puromycin, PLGRKT, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256789pLKO HA-2A-Cre v4 trcr fillerlentiviral construct with HA-2A-Cre recombinase and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256790pLKO Cre-2A-Plgrkt v4 trcr fillerlentiviral construct with Cre recombinase-2A-Plgrkt and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256791pLKO Cre-2A-Plgrkt K147R v4 trcr fillerlentiviral construct with Cre recombinase-2A-Plgrkt K147R and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
256792pLKO Cre-2A-Plgrkt Δ139-147 v4 trcr fillerlentiviral construct with Cre recombinase-2A-Plgrkt Δ139-147 and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.

Antibodies from Article