pLKO H2BmRFP-2A-Plgrkt Δ139-147 v4 trcr filler
(Plasmid
#256784)
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Purposelentiviral construct with H2BmRFP-2A-Plgrkt (mouse) Δ139-147 and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLKO H2BmRFP-2A-puro v4 trcr filler
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePlgrkt
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SpeciesM. musculus (mouse)
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Mutationdelta139-147
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Entrez GenePlgrkt (a.k.a. 1110007H22Rik, 5033414D02Rik, Plg-R(KT), Plg-RKT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO H2BmRFP-2A-Plgrkt Δ139-147 v4 trcr filler was a gift from Daniel Schramek (Addgene plasmid # 256784 ; http://n2t.net/addgene:256784 ; RRID:Addgene_256784) -
For your References section:
Aneuploidy selects for the acquisition of driver genes in breast cancer. Al-Zahrani KN, Langille ER, Nurtanto J, Obersterescu A, Teng K, Lowden C, Dessapt J, Chiu CH, Caldwell LV, Cook DP, Perez-Castro MA, Berman JM, Tsai R, Bahcheli AT, Mbamalu G, Wu S, Narimatsu M, Lopes AG, Fotiadou I, Chan K, Zhang L, Bang KWA, Parsons MJ, Mourao L, Temel EI, McCulla L, Sravya P, Zhang L, Sajjakulnukit P, Lyssiotis CA, Borowsky AD, Scheele CLGJ, Wahl DR, Jackson HW, Stewart KS, Fuchs E, Egan SE, Pujana MA, Reimand J, Wrana JL, Schramek D. Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10752-9. 10.1038/s41586-026-10752-9 PubMed 42420452