pLKO Cre-2A-Plgrkt K147R v4 trcr filler
(Plasmid
#256791)
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Purposelentiviral construct with Cre recombinase-2A-Plgrkt K147R and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLKO-Cre stuffer v4
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePlgrkt
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SpeciesM. musculus (mouse)
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MutationK147R
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Entrez GenePlgrkt (a.k.a. 1110007H22Rik, 5033414D02Rik, Plg-R(KT), Plg-RKT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO Cre-2A-Plgrkt K147R v4 trcr filler was a gift from Daniel Schramek (Addgene plasmid # 256791 ; http://n2t.net/addgene:256791 ; RRID:Addgene_256791)