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eGFP-MKRN2-dRING-pcDNA4/TO
(Plasmid #257246)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257246 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA4
  • Total vector size (bp) 7209
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP-MKRN2--dRING
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2013
  • Entrez Gene
    MKRN2 (a.k.a. HSPC070, RNF62)
  • Tag / Fusion Protein
    • eGFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.15.682570 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP-MKRN2-dRING-pcDNA4/TO was a gift from Eran Hornstein (Addgene plasmid # 257246 ; http://n2t.net/addgene:257246 ; RRID:Addgene_257246)

  • For your References section:

    The E3 ligase MKRN2 prevents DRiP accumulation in stress granules and maintains granulostasis. Amzallag E, Danino YM, Secco V, Carra S, Hornstein E. EMBO Rep. 2026 Jun 15. doi: 10.1038/s44319-026-00832-2. 10.1038/s44319-026-00832-2 PubMed 42297957
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