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pAH160_Tn_AIcrVIA3_v2
(Plasmid #257248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257248 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAH160
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AIcrVIA3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RSF1030-based Himar1 transposon donor plasmid carrying the AIcrVIA3 anti-CRISPR cassette under the constitutive synthetic promoter BBa_J23110, flanked by Himar1 inverted repeats (IRs). Transposition is mediated by the Himar1 transposase gene expressed from a separate plasmid. Please visit https://doi.org/10.1101/2025.11.20.689424 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAH160_Tn_AIcrVIA3_v2 was a gift from Alexander Harms (Addgene plasmid # 257248 ; http://n2t.net/addgene:257248 ; RRID:Addgene_257248)

  • For your References section:

    Systematic mapping of bacteriophage gene essentiality with HIDEN-SEQ. Humolli D, Piel D, Ransome J, Bausch K, Tschudin-Sutter S, Ortelli M, Dehio C, Veening JW, Harms A. Nat Microbiol. 2026 Sep 25. doi: 10.1038/s41564-026-02455-8. 10.1038/s41564-026-02455-8 PubMed 42791354
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