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Systematic mapping of bacteriophage gene essentiality with HIDEN-SEQ.

Humolli D, Piel D, Ransome J, Bausch K, Tschudin-Sutter S, Ortelli M, Dehio C, Veening JW, Harms A
Nat Microbiol. 2026 Sep 25. doi: 10.1038/s41564-026-02455-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
257247pAH186ColE1_Himar1_T’asePlasmid carrying the Himar1 transposase gene.
257249pDH160_Tn_AcrVIA1_v2Transposon plasmid carrying the AcrVIA1 gene expressed from a strong synthetic promoter BBa_J23119.
257250pAH221_LbuCas13aLbuCas13a-expressing plasmid.
257251pAH218_LbuCas13a_eLbuCas13a crRNA entry vector.
257252pAH221_LseCas13aLseCas13a-expressing plasmid.
257253pAH218_LseCas13a_eLseCas13a crRNA entry vector.

Antibodies from Article