pMCSG53_ K9F3F4_UCL44
(Plasmid
#257302)
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PurposeBacterial expression of acetyltransferase from Leptolyngbya sp.
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Depositing Lab
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Depositing OrganizationSan Francisco State University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMCSG53
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Backbone manufacturerArgonne National Laboratory
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameacetyltransferase
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Alt nameK9F3F4
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Alt nameLepto7375DRAFT_4928
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SpeciesLeptolyngbya sp. PCC 7375
- Promoter T7
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sequences of inserts are designed to be subcloned via LIC into ssp1 site of vector. For gene/insert information, visit https://www.uniprot.org/uniprotkb/K9F3F4/entry.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSG53_ K9F3F4_UCL44 was a gift from Misty Kuhn (Addgene plasmid # 257302 ; http://n2t.net/addgene:257302 ; RRID:Addgene_257302) -
For your References section:
Structural plasticity drives convergent evolution and functional diversification of GNAT polyamine acetyltransferases. Roca-Martinez J, Leiva Martel HN, Yin J, Do HD, Le VTB, Bordin N, Rauer C, Orengo C, Kuhn ML. Structure. 2026 Aug 24:S0969-2126(26)00246-7. doi: 10.1016/j.str.2026.07.015. 10.1016/j.str.2026.07.015 PubMed 42636798