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pMCSG7_ Q9KL03_UCL56/VcSpeG
(Plasmid #257314)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257314 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pMCSG7
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VcSpeG
  • Alt name
    Q9KL03
  • Species
    Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
  • Promoter T7

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequences of inserts are designed to be subcloned via LIC into ssp1 site of vector. For gene/insert information, visit https://www.uniprot.org/uniprotkb/Q9KL03/entry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG7_ Q9KL03_UCL56/VcSpeG was a gift from Misty Kuhn (Addgene plasmid # 257314 ; http://n2t.net/addgene:257314 ; RRID:Addgene_257314)
  • For your References section:

    Structural plasticity drives convergent evolution and functional diversification of GNAT polyamine acetyltransferases. Roca-Martinez J, Leiva Martel HN, Yin J, Do HD, Le VTB, Bordin N, Rauer C, Orengo C, Kuhn ML. Structure. 2026 Aug 24:S0969-2126(26)00246-7. doi: 10.1016/j.str.2026.07.015. 10.1016/j.str.2026.07.015 PubMed 42636798