PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25739||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerIain Fraser
- Backbone size w/o insert (bp) 12370
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameLuciferase miR-shRNA
/ Fusion Protein
- Venus (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BfuA1 (unknown if destroyed)
- 3′ cloning site BfuA1 (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EXFP-R, hUBCpro-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pSLIK-Venus with TRE-driven Luciferase
Luciferase shRNA sequence is - 5'- CCCGCCTGAAGTCTCTGATTAATAGTGAAGCC ACAGATGTATTAATCAGAGACTTCAGGCGGT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSLIK-Venus-TmiR-Luc was a gift from Iain Fraser (Addgene plasmid # 25739 ; http://n2t.net/addgene:25739 ; RRID:Addgene_25739)
For your References section:A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906