PB-EF1a-ERK-phosphoGal4v4a-IRES-mCherry
(Plasmid
#257515)
-
PurposeAn ERK-responsible Gal4-VP64 transcription factor. Created by allosteric insertion of a kinase-controlled protein switch
-
Depositing Lab
-
Depositing OrganizationPrinceton University
Located in United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257515 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepiggyBac
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameERK-phosphoGal4v4a-IRES-mCherry
- Promoter EF1a
Cloning Information
- Cloning method Other
- 5′ sequencing primer ACGCGtcaagtGGAGCAAGGCA
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.04.15.648940 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PB-EF1a-ERK-phosphoGal4v4a-IRES-mCherry was a gift from Jared Toettcher (Addgene plasmid # 257515 ; http://n2t.net/addgene:257515 ; RRID:Addgene_257515) -
For your References section:
Design and optimization of a kinase-controlled allosteric switch. Cao Q, Toettcher JE. Nat Methods. 2026 Aug;23(8):1540-1552. doi: 10.1038/s41592-026-03163-1. Epub 2026 Jul 21. 10.1038/s41592-026-03163-1 PubMed 42481857
