Design and optimization of a kinase-controlled allosteric switch.
Cao Q, Toettcher JE
Nat Methods. 2026 Aug;23(8):1540-1552. doi: 10.1038/s41592-026-03163-1. Epub 2026 Jul 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 257515 | PB-EF1a-ERK-phosphoGal4v4a-IRES-mCherry | An ERK-responsible Gal4-VP64 transcription factor. Created by allosteric insertion of a kinase-controlled protein switch |
| 257516 | PB-EF1a-ERK-phosphoGal4v4a(TA)-IRES-mCherry | A control ERK-responsible Gal4-VP64 transcription factor with phospho-site mutation. A T-to-A mutation is introduced in the ERK substrate motif in the phospho-switch |
| 257517 | pHR-SFFV-ERK-phosphoActinNanobodyv3-tagRFP | An ERK-responsible F-actin nanobody. Created by allosteric insertion of a kinase-controlled protein switch |
| 257518 | CMV-mCherry-ERK-phosphoVav2 | An ERK-responsible Vav2. Created by allosteric insertion of a kinase-controlled protein switch |
| 257520 | PB-EF1a-JNK-phosphoGal4v3-IRES-mCherry | A JNK-responsible Gal4-VP64 transcription factor. Created by allosteric insertion of a kinase-controlled protein switch |