CMV-mCherry-ERK-phosphoVav2
(Plasmid
#257518)
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PurposeAn ERK-responsible Vav2. Created by allosteric insertion of a kinase-controlled protein switch
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Depositing Lab
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Depositing OrganizationPrinceton University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepmCherry
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry-ERK-phosphoVav2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.04.15.648940 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-mCherry-ERK-phosphoVav2 was a gift from Jared Toettcher (Addgene plasmid # 257518 ; http://n2t.net/addgene:257518 ; RRID:Addgene_257518) -
For your References section:
Design and optimization of a kinase-controlled allosteric switch. Cao Q, Toettcher JE. Nat Methods. 2026 Aug;23(8):1540-1552. doi: 10.1038/s41592-026-03163-1. Epub 2026 Jul 21. 10.1038/s41592-026-03163-1 PubMed 42481857