pUC19 (91nt-gap) (NN3)
(Plasmid
#257528)
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PurposeTo create a 91nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI, Nb.BsmI, and Nt.BspQI. Excised ssDNA are 22-24 bases.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC19
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Vector typeNot for gene expression. A DNA template for biochemical studies.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNb.BbvCI, Nb.BsmI, and Nt.BspQI nicking sites
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19 (91nt-gap) (NN3) was a gift from Michael O'Donnell (Addgene plasmid # 257528 ; http://n2t.net/addgene:257528 ; RRID:Addgene_257528)