Michael O'Donnell Lab: O'Donnell Lab Plasmids 2026 (Batch 10)
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 257522 | Sc FLAG-Pol1(STS)-pRS402/GAL-L | Overexpress Sc FLAG-Pol1(STS) in S. cerevisiae when integrated into the genome |
| 257524 | pUC19 (4nt-gap) (NN44) | To create a 4nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI & Nb.BsmI |
| 257526 | pUC19 (8nt-gap) (NN48) | To create an 8nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI & Nb.BsmI |
| 257527 | pUC19 (10/20nt-gap) (NN1) | To create a 10nt single-stranded gap by nicking with (Nb.BbvCI & Nb.BsmI) or (Nb.BsmI & Nt.BspQI) or a 20nt single-stranded gap by nicking with Nb.BbvCI, Nb.BsmI, and Nt.BspQI |
| 257528 | pUC19 (91nt-gap) (NN3) | To create a 91nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI, Nb.BsmI, and Nt.BspQI. Excised ssDNA are 22-24 bases. |
| 257529 | Ec dnaA-pET(11a) | Overexpress Ec dnaA in E. coli |
| 257530 | Ec dnaG-pET(3d) | Overexpress Ec dnaG in E. coli |